Tris have been useful as buffers in a wide variety of biological systems. It has been used as a starting material for polymers, oxazolones (with carboxylic acids) and oxazolidines (with aldehydes). It does not precipitate calcium salts and is of value in maintaining solubility of manganese salts.…

PIPES is a zwitterionic N-substituted aminosulfonic acid "Good" buffer. PIPES is used to buffer glutaraldehyde fixative solutions when fixing plant and/or animal tissue samples. This will help prevent the loss of lipids. It is also used to buffer glutaraldehyde and formaldehyde when…

Tris-HCl is an excellent biochemical and biological buffer for all applications where ultra high purity is required. The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05…

HEPES is a zwitterionic organic chemical buffering agent; one of the twelve Good's buffers. HEPES has been utilized extensively for research and commercial applications in the cell culture and tissue culture industries. HEPES is widely used in cell culture, largely because it is better at…

Boric acid has a single boron; its salt forms complex equilibria in solution, with the ion B4O7-2 being the predominant species. Boric acid is used for weatherproofing wood and fireproofing fabrics; as a preservative; manufacturing of cements, crockery, porcelain, enamels, glass, borates,…

Potassium phosphate is a reagent with high buffering capacity. It occurs in several forms: monobasic, dibasic, and tribasic (K3PO4). Most pH neutral potassium phosphate buffer solutions consist of mixtures of the monobasic and dibasic forms to varying degrees, depending on the desired pH. …

Potassium phosphate is a reagent with high buffering capacity. It occurs in several forms: monobasic, dibasic, and tribasic (K3PO4). Most pH neutral potassium phosphate buffer solutions consist of mixtures of the monobasic and dibasic forms to varying degrees, depending on the desired pH. …

Sodium phosphate monobasic is a reagent with very high buffering capacity widely used in molecular biology, biochemistry and chromatography. Useful in conjunction with Sodium Dibasic Phosphate, in the preparation of biological buffers. Used in many applications including the purification of antibodies.

Bis-Tris is a zwitterionic buffer; an amine buffer similar to Tris Hydrochloric acid that exhibits only a very small shift in dissociation constant with respect to temperature. It is structurally analogous to the the Good buffers that were developed to provide buffers in the pH range of 6.15 - 8.35…

MOPS is a zwitterionic amino acid which acts as one of the "Good" buffers. Concentrations higher than 20 mM should not be used with mammalian cell cultures.

Tricine, a zwitterionic buffer, was first prepared by Good for use as a buffer for chloroplast reactions. It is structurally similar to Tris, but is much less inhibitory at high concentrations. The name tricine comes from tris and glycine from which it was derived. Tricine can be used in…

MES is a zwitterionic buffer. One of the "Good" buffers developed for biological applications. It has the advantages of maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pK with temperature, chemically and enzymatically stable,…

Sodium dibasic anhydrous is used as a sequestrant, emulsifier and buffer in foods, as mordant in dyeing for weighting silk, in tanning, in manufacturing of enamels, ceramics, detergents, boiler compounds; as fireproofing agent in soldering and brazing instead of borax; as reagent and buffer in…

Tris has been useful as buffers in a wide variety of biological systems. Uses include pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications. Tris has been used as a starting material for polymers, oxazolones (with carboxylic acids) and…

MOPS is used as a biological buffer. MOPS is a zwitterionic amino acid which acts as one of the "Good" buffers. It is a structural analog to MES; however, MOPS is a more suitable biological buffer than MES because the pH range is closer to pH 7.4. A buffer using MOPS sodium salt can be…

Triethanolamine is a zwitterionic buffer. Triethanolamine(TEA) is used primarily as an emulsifier and surfactant. It neutralizes fatty acids, adjusts and buffers the pH, and solubilises oils and other ingredients that are not completely soluble in water. Triethanolamine is also used as organic…

HEPPS is used as a separator in ultrathin isoelectric focusing gels; enhances the resolution of phosphoglucomutase. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration).

MOPS is a zwitterionic amino acid which acts as one of the "Good" buffers. It is a structural analog to MES. However, MOPS is a more suitable biological buffer than MES because the pH range is closer to pH 7.4.

Agar is a polysaccharide complex extracted by bleaching and hot water treatment of agarocytes from the red alga Rhodophyceae, and usually consisting of the genera Gelidium, Acanthopeltis, Ceramium,Pterocladia and Gracilaria. The algae are typically found in the Pacific and Indian Oceans and in the…

HEPES is a zwitterionic organic chemical buffering agent; one of the twelve Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to…

Bis-Tris is zwitterionic buffer, an amine buffer similar to Tris. It exhibits only a very small shift in dissociation constant with respect to temperature. Hydrochloric acid can be used to adjust pH for a Bis-Tris buffer to the desired pH.

PIPES is a zwitterionic N-substituted aminosulfonic acid "Good" buffer. Buffers can be made by adding a solution of base to PIPES free acid, titrating to the appropriate pH, or by mixing equimolar solutions of the sesquisodium salt and the disodium salt or dipotassium salt, titrating to…

HEPES is a zwitterionic organic chemical buffering agent; one of the twelve Good's buffers. HEPES is widely used in cell culture largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to…

BES is a zwitterionic buffer that is used in biochemistry and molecular biology research in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies. A protocol has been reported for the use of BES buffered saline in the calcium phosphate mediated transfection of eukaryotic cells…

Tricine was first prepared by Good for use as a buffer for chloroplast reactions. It is structurally similar to Tris, but is much less inhibitory at high concentrations. For ATP assays using firefly luciferase, tricine buffer at 25 mM was found to be the best of ten common buffers tested. …

MES is a zwitterionic buffer. One of the "Good" buffers developed for biological applications. It has the advantages of: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pK with temperature chemically and…

Tris is an excellent biochemical and biological buffer for all applications where ultra high purity is required.

Tris and Tris Hydrochloride have been useful as buffers in a wide variety of biological systems. Uses include pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications. Tris has been used as a starting material for polymers, oxazolones (with…

Borax anhydrous is typically used as a buffer component. The Merck Index lists other uses as: used in the manufacturing of glazes and enamels, a component in tanning agents; curing and preserving skins, an agent used in cleaning compounds, used to artificially age wood, as a preservative, either…

Used as a buffer in biological research. HEPPSO is an efficient separator in protein resolution by IEF. HEPPSO is a Zwitterionic hydrogen buffer.

HEPES is a zwitterionic organic chemical buffering agent. HEPES is widely used in many biochemical reactions and as a buffering agent in some cell culture media.

Tris is relatively non-hygroscopic; however, if needed, Tris Base can be dried at 100°C for up to 4 hours to remove any water. Neither Tris Base or Tris Hydrochloride by themselves provide adequate buffering capacity. Generally the two need to be mixed together to provide a buffer with pH…