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2D Sample Preparation

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  • DeStreak™ Reagents

    GE Healthcare

    Improves reproducibility and quality of 2-D gels by preventing streaking Eliminates extra spots caused by nonspecific oxidation of proteins Stabilizes proteins throughout entire 2-D runs, ensuring simplified, well-resolved protein patterns Highly suitable for both analytical and…

  • PlusOne™ Dithiothreitol (DTT)

    GE Healthcare

    Dithiothreitol (DTT) at a concentration of 1% is used to reduce disulphide bonds for electrophoretic analysis of protein subunits. At low concentrations (1 mM) dithiothreitol is used to counteract the oxidation of proteins and preserve biological activity.

  • Iodoacetamide

    GE Healthcare

    Ultrapure, proteomic-grade iodoacetamide is used for efficient alkylation of thiols while minimizing oxidation of the competing thiol pairs in protein samples.

  • PlusOne™ CHAPS

    GE Healthcare

    CHAPS (3-((3-cholamidopropyl)dimethylammonio)-1- propanesulfonate) is used for protein solubilization during sample preparation for 2-D electrophoresis. CHAPS is a zwitterionic detergent with the ability to disrupt non-specific protein interactions and preserve the native form of proteins. CHAPS…

  • PlusOne™ Sodium Dodecyl Sulfate (SDS)

    GE Healthcare

    The quality of SDS is of utmost importance for good electrophoretic results. Impurities with long carbon chains will influence micelle size and thus the migration of the proteins. A long carbon chain will also make staining difficult as impurities will adsorb to proteins and prevent binding of…

  • Thiourea

    GE Healthcare

    Thiourea is a chaotrope used together with urea in the IPG strip rehydration to solubilize and denature proteins, and improve the solubility of membrane proteins.

  • Immobiline DryStrip Cover Fluid

    GE Healthcare

    This specially purified fraction of mineral oil protects immobiline drystrip gels from drying and from the atmosphere during the first dimension of 2-D electrophoresis. The fluid is also suitable as a contact between cooling plate and gel backing film in horizontal electrophoresis. 330°C…

  • PlusOne™ Urea

    GE Healthcare

    PlusOne quality urea is specially suitable for manual DNA sequencing, where separations are often run at high concentrations of urea. It is also used to improve the solubility of hydrophobic proteins close to their isoelectric points, especially in IEF. IEF performed under denaturing conditions…

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