• Isolated from calf intestine

 • Hydrolysis of 5’ residues

 • Active on RNA and DNA

 • Heat Inactivated

Calf intestine alkaline phosphatase (CIP) hydrolyzes 5’-terminal monophosphate groups of DNA and RNA and 5’-di- and triphosphate groups of RNA. This enzyme is widely used for removing 5’ phosphates from fragments of DNA or RNA prior to labelling the 5’ end with polynucleotide kinase, or to prevent self-ligation of cloning vectors.
Unlike BAP, CIP is inactivated by heat. The enzyme has been tested for the absence of nickase, ribonuclease, endo- and exonuclease activity.