• Transform-It™ Solution is ready-to-use

 • Transform Plasmids, Ligations, and Cosmids

 • No Heat Shock Required

 • Store Competent Cells 10 weeks or longer

 • Easy procedure with minimal “hands-on” time

The Transform & Grow Bacterial Transformation Kit provides a simple and efficient method for the preparation, transforma-tion and storage of competent E. coli cells. Bacterial cells are grown to mid-log phase at 37°C, pelleted and resuspended in Transform-It Solution. This buffer enables the cells to become immediately available for transformation and permits freezing the cells for future use without further preparation. The competent cells remain stable at
-70°C for at least 10 weeks.
The Transform & Grow Bacterial Transformation Kit eliminates the requirement for heat shock and prepares competent cells within two and a half hours with only 30 minutes of hands-on manipulation. The kit includes Transform-It Solution, SUPER-COMP Media and Roll & Grow™ Plating Beads to prepare competent cells with a reliable and reproducible transformation efficiency of 2—8 x10⁶ transformants per µg of supercoiled plasmid DNA.
Reagents for 50 transformations are provided.