• High level expression vectors

A variety of sgGFP/sgBFP encoding vectors are available, optimally designed for numerous applications. CMV promoter-driven AFP® are the choice for optimal transient expression in mammalian cells as well as for the generation of AFP-fusion constructs or promoter evaluation.
AFP-neomycin fusions driven by either murine pgk or pol II promoters offer dual selection possibilities for the rapid evaluation of transfection efficiencies and for establishing stably transformed fluorescent cell-lines. In addition, localization vectors that express fusion proteins confined to various subcellular compartments are also available.
All vectors are designed to feature:
AFP’s with mutations that optimize protein folding and fluorophore formation at 37°C.
Codon-optimized coding sequences to stabilize mRNA structure and enhance expression without restricting host range.