Two monoclonal antibodies (mAb) are offered for the detection of Qbiogene’s SuperGlo™ GFP and SuperGlo™ BFP. Both also recognize all other variants. Anti-AFP® mAbs are purified from the supernatant of mouse hybridomas.
The 11E5 mAb, which is a mouse IgG1 monoclonal antibody recognizing GFP and other variants in their denatured form, is ideal for detection in Western analyses. Use of the 11E5 mAb allows detection of as little as 10 ng of GFP using colorimetric methods, and will detect picogram quantities with chemiluminescent detection assays. Performing Western blots with the 11E5 mAb allows confirmation of AFP or AFP-fusion protein expression at the expected molecular weight.
The 3E6 mAb, an IgG2a antibody recognizing the native form of GFP, can be used for immuno-precipitation, immunocytochemistry, and ELISA assays. Purification of AFP and AFP- fusion proteins is possible using either protein A or protein G.
Both antibodies are highly specific for GFP and its variants in both bacterial and mammalian cell extracts, giving strong, clean signals.