• Broadest Stoke’s shift of all green variants

 • Single enhanced excitation peak

Mutations present in sgGFP™:

 • F64L: improved solubility, and protein folding at 37°C; more rapid and efficient fluorophore formation

 • S65C: a single excitation peak at 474 nm and emission at 509 nm

 • 1167T: enhanced excitation peak at 474 nm

sgGFP™ SuperGlo™ Green Fluorescent Protein is the GFP with a single
enhanced excitation peak at 474 nm and a green fluorescence at ~ 509 nm. All other popular variants have excitation peaks at or near 488 nm. sgGFP™ is the only GFP that has a Stoke’s shift that exceeds the minimum of 30 nm recommended for easy visualization and sensitive detection. sgGFP can be readily detected in fluorimeters, fluorescent cell sorters (FACS) and by fluorescent microscopes. sgGFP will also partner for FRET with sgBFP.