4313U25 series

Polyvinylidene Fluoride Transfer Membrane

 • Naturally hydrophobic

 • Unsupported transfer membrane

 • Reproducible, consistent results with maximum sensitivity

Naturally hydrophobic, unsupported polyvinylidene fluoride transfer membrane is designed to deliver the highest binding capacity combined with low backgrounds in western transfer, amino acid analysis and protein sequencing applications. Exceptional quality control procedures throughout the manufacturing process eliminates "lot-to-lot" variation and minimizes "bald spot" problems. PVDF-Plus assures reproducibe results. Protein can be electroblotted from a variety of gel matricies onto PVDF-Plus. Because of its high sensitivity and strong binding affinity, protein will not pass through the membrane.

The chemical compatibility of PVDF-Plus allows the use of all commonly used stains such as: Amido Black, Colloidal Gold, Coomassie Blue, India Ink and Ponceau-S. Will not degrade, distort or shrink when using high concentrations of methanol for destaining. Exhibits efficient transfer of proteins in a wide range of molecular weights, from 5k daltons to 700k daltons. The binding capacity averages 125 µg/cm² for larger globular proteins such as immunoglobulins and higher binding capacities for small peptides. All membranes have 0.45 µm pore sizes. Available in sheets and rolls.