For Customer Service Call 1.800.345.2100 International Sales
Delivering Quality Scientific Supplies Worldwide Since 1900
BD BioCoat™ Cell Culture Inserts contain BD Falcon™ Cell Culture Inserts coated with extracellular matrix (ECM) and are packaged ready-to-use in BD Falcon™ Companion Plates. Cell culture on microporous membranes has been shown to promote differentiation of a variety of epithelial and mesenchymal cells in vitro, especially in the presence of ECM.
ECM is a vital component of the cellular microenvironment in vivo. The use of ECM growth substrates in vitro has been shown to promote cell growth, cell-specific morphology, and differentiated function. For example, collagen I and fibronectin have been used on microporous inserts to establish barrier function in monolayers of intestinal epithelial and endothelial cells, to culture a renal cell line that expresses kidney-specific peptide transporters, and to culture keratinocytes. Additionally, BD BioCoat™ Matrigel™ Matrix coated inserts have been used to maintain polarized secretion in uterine, thyroid and mammary epithelial cells.
The track-etched polyethylene teraphthalate (PET) membranes used in BD BioCoat™ Cell Culture Inserts have individual cylindrical pores in a broad range of sizes including 0.4, 1.0, 3.0, and 8.0 µm.
The PET membrane offers several advantages:
BD BioCoat™ Cell Culture Inserts offer:
BD BioCoat™ Cell Culture Inserts are available with the following selection of ECMs: Three-Dimensional Matrices: BD Matrigel™ Matrix, Fibrillar Collagen Thin-Layer Matrices: Collagen I, Fibronectin, Collagen IV, Laminin
Quality Control: All cell culture inserts are tested for ability to promote cell attachment and spreading or differentiation (cell type used is specific for each ECM). Tested and found negative for bacteria and fungi.
Storage and Stability: BD BioCoat™ Collagen I, Collagen IV, Fibrillar Collagen, Fibronectin, and Laminin Inserts stable for = three months at 2-8°C. BD BioCoat™ Matrigel™ Matrix Inserts stable for = three months at -20°C. BD BioCoat™ Control Inserts and Deep-Well Plates stable at room temperature.
Thomas Scientific is a Small Business, EEO supplier ©Thomas Scientific 2013