Optimized for Basic Research and Drug Discovery Applications
BD PureCoat™ amine and carboxyl surfaces are proven to provide improved attachment, increased proliferation, enhanced recovery from freeze-thaw, and improved differentiation for a broad range of cells that exhibit poor attachment properties in standard, serum-free or serum-reduced conditions (e.g., primary cells, transfected cells, and fastidious cell lines). In drug discovery assays, when using transfected cells and commercially prepared division arrested cell lines, both surfaces maintain cell monolayers during vigorous liquid handling manipulation.
BD PureCoat™ surfaces are a novel family of chemically-defined, animal-free cell culture surfaces designed to enhance cell performance. A proprietary thin-film coating technology produces a uniform, functionalized surface that provides a highly controlled environment for cell culture applications.
Both BD PureCoat™ amine, a positively charged surface, and BD PureCoat carboxyl, a negatively charged surface, are proven to provide improved cell attachment and cell proliferation over standard tissue culture (TC)-treated surfaces. These defined surfaces function with a broad range of primary and transfected cells in standard, serum-free, or serum-reduced culture conditions. BD PureCoat surfaces support standard cell dissociation techniques, do not interfere with microscopy or imaging analysis, and do not require special steps for cell adaptation.
Defined Animal-Free Cell Culture Surfaces
BD PureCoat™ amine and carboxyl surfaces are chemically-defined, animal-free enhanced cell culture surfaces. Both surfaces reduce variability, giving you more control over your cell culture environment and resulting in a more predictable biological outcome.
Improved Attachment and Increased Proliferation
BD PureCoat™ surfaces provide improved attachment, increased proliferation, enhanced recovery from freeze-thaw, and improved differentiation for a broad range of cells that exhibit poor attachment properties. These features save you time while increasing productivity in the lab.
| | Amine (+) | Carboxyl (-) |
Primary Neuronal Cells Rat
Brain Cortex | Yes | |
| Rat Cerebellar Granule (RCG) | Yes | |
Primary Cells Human Epidermal
Keratinocytes (Neonatal)* | Yes | |
| Human Placental Epithelial Cells* | Yes | |
| Primary Cervical Epithelial Cells* | Yes | |
| Rat Astrocytes | Yes | |
| Rat and Mouse* Cardiomyocytes | | Yes |
| Rat Epidermal Keratinocytes | | Yes |
| Rat Primary Pancreatic Islet* | Yes | |
Stem Cells Embryonic Mouse
Brain Stem Cell* | Yes | |
| Human Mesenchymal Stem Cells* | Yes | |
| MSC (Rat bone marrow derived)* | Yes | |
Rat E14d Cortex Derived
Neural Stem Cells* | Yes | |
| Transfected Cells 293T* | Yes | |
| EcoPack™2-293 | Yes | Yes |
| Flp-In™ T-REx™ 293* | Yes | |
hERG-T-REx™ 293 Division
Arrested Cells | Yes | |
Living Colors™ HEK-ZsGreen
Proteasome Sensor | Yes | Yes |
Cell Lines Baby Hamster
Kidney (BHK-21) | Yes | |
| CHO | Yes | |
| HEK-293 | Yes | Yes |
| HeLa* | | Yes |
| HepG2 | Yes | Yes |
| HT-1080 | | Yes |
| LnCAP | | Yes |
| MRC-5 | | Yes |
| N2A* | Yes | |
| PC12 | Yes | Yes |
*Cells successfully cultured by BD customers on BD PureCoat surfaces