GenScript AmMag™ Protein A Magnetic Beads are super paramagnetic beads with average diameter of 50 µm, covalently coated with alkali tolerant protein A. Protein A is a bacterial cell wall protein isolated from Staphylococcus aureus, which binds to mammalian IgG’s mainly through the Fc regions. The alkali tolerant protein A not only retains the specific binding capacity to Fc region of immunoglobulin molecules, but additionally tolerates alkaline conditions and can withstand rigorous cleaning procedures with 0.1 M NaOH.
The beads are supplied as 25% slurry in 20% ethanol. The AmMag™ protein A Magnetic Beads have a binding capacity of 40 mg human IgG per 1 ml settled beads. They can be reused upon washing with 0.1 M NaOH.
The sample containing antibody is added to the AmMag™ Protein A Magnetic Beads and incubated on a shaker for a short time for the antibody to bind to the beads. The antibody bound to the beads can be eluted off of the beads using acidic elution facilitated by a magnetic separation device. Magnetic separation eliminates the need for centrifugation, minimizes the loss of sample and makes the process more user friendly.