Total RNA Minipreps from Plant and Fungal Tissues
The Total RNA MINI and MAXI Kits (Plant) provide a simple and fast method to isolate total RNA from plant tissue and cells. Samples are ground in liquid nitrogen and filtered to remove debris. In the presence of a binding buffer and chaotropic salt, the total RNA in the lysate binds to the glass fiber matrix of the spin column. The optional DNase treatments can remove DNA residues and the contaminants can be washed with an ethanol based wash buffer. Finally, the purified total RNA is eluted by RNase-Free water. This protocol does not require phenol extraction or alcohol precipitation, and the entire procedure can be completed within 60 minutes. The purified total RNA is ready for RT, RTPCR, Real-Time PCR, and Northern Blotting.