The Direct-zol RNA Miniprep provides a streamlined method for the purification of up to 50 µg (per prep) of high-quality RNA directly from samples in TRI Reagent or similar. Total RNA (including small and non-coding RNAs) is effectively isolated from a variety of sample sources (cells, tissues, biological liquids, etc.) using this product.
The procedure is easy: simply apply a sample in TRI Reagent to spin column and then spin, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. Small RNAs (≥17 nucleotides) are efficiently and consistently recovered using this kit. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc.
The entire procedure typically takes about 5-10 minutes.