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ACTGene, Inc.

ACTaq™ Hot-Start DNA Polymerase

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ACTaq™ DNA Polymerase originates from Thermus Aquaticus with molecular weight of 94KDa. ACTaq™ Hot-Start DNA Polymerase differs from ACTaq™ DNA Polymerase such that it contains antibody binding Taq which enables the activation of Taq only at 95oC. The activation step eliminates the presence of non-specifics such as primer-dimer formation and mis-primed products, by ensuring the enzyme is inactive at temperature below 95oC. ACTaq™ Hot-Start DNA Polymerase reduces risk of contamination, making reaction preparation at ease when carried out in room temperature.

Hot-Start Activation Step:pre-incubate 5 minutes at 95°C prior to thermal cycling
Storage Condition:-20°C. Stable for 12 months in constant freezer temperatur
Concentration:5 Units/μL
Unit Definition:One unit of the amount of enzyme that incorporates 10nmols of dNTPs into
acid-insoluble material in 30 minutes at 72°C
Product Components:ACTaq™ Hot-Start DNA Polymerase (Antibody bound recombinant Taq DNA Polymerase); PCR reaction buffer; 10X 25mM MgCl2
Storage Buffer:10mM Tris (pH 8.2), 50mM KCL, 0.5% BSA, 0.5% NP-40, 50% glycerol
PCR Reaction Buffer:10x PCR buffer with Mg2+. 15mM MgSO4, 100mM KCl, 80mM
(NH4)2SO4, 100mM Tris-HCl, pH 9.0, 0.5%NP-50
Quality Control:ACTaq™ Hot-Start DNA Polymerase is highly purified. Free of contaminating
endonucleases, exonucleases, and nicking activities. The purity of the
enzyme is evaluated by SDS-PAGE at >95% purity.

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Product Detail
Thomas No.
C995K12
Mfr. No.
E3500-250U
Description
ACTaq™ Hot-Start DNA Polymerase, 250Units/Vial
list price/quantitytotal
$0.00
Thomas No.
C995K13
Mfr. No.
E3500-1000U
Description
ACTaq™ Hot-Start DNA Polymerase, 1000Units/Vial
list price/quantitytotal
$0.00
$0.00 (0 Items)
Product is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

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