Casitose Yeast Extract Broth (CAYE) is recommended for cultivation of Vibrio cholerae cultures while testing their enterotoxigenicity. This medium is formulated as per APHA for cultivating Vibrio cholerae while testing their enterotoxigenicity as these media enhance the production of Vibrio enterotoxin. The medium contents like casein acid hydrolysate and yeast extract provide the essential nitrogenous nutrients and B-vitamins to the growing Vibrio's.