Chlorella Broth is recommended for the cultivation & enumeration of Chlorella species. This medium was originally formulated by Shrift and further modified for cultivation and maintenance of Chlorella. Chlorella being photosynthetic green algae, should be cultivated in the presence of light. Bright diffused light, fluorescent light and sunlight are satisfactory sources of light for the growth of Chlorella. The inoculated tubes/flasks should be incubated in the presence of light at 25-27deg C for a week to permit good growth and pigmentation. Chlorella cultures can be maintained at room temperature for 2-3 months without sub culturing.