Tissue Culture Media
The primary purpose of tissue culture media is to provide a synthetic environment within the lab. This synthetic environment then allows cells to thrive and carry out normal functions.
Ideal for primary cell isolation and cell line maintenance, culture media is essential for the isolation and propagation of primary cells obtained from tissues.
PBS cell culture media is recommended for use when investigating the localization and distribution of target proteins in different parts of biological tissue.
Tissue culture media accessories include reagent-grade water, antibiotics, and Corning BioCoat cell culture inserts to assist you with achieving clear and accurate results.
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Dulbecco's Modified Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the amino acids and vitamins found in BME. The original formulation was used to culture embryonic mouse cells and has since been modified to support a variety of…
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Dulbecco's Phosphate Buffered Saline (DPBS) is a buffer solution which can be used to maintain cell culture media in the physiological pH range of 7.0 to 7.6. It can serve as an irrigating, transporting or diluting fluid while maintaining cell tonicity and viability for a limited period of…
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Cell Dissociation
CorningTrypsin is used to enzymatically release adherent cells from tissue culture plates for passaging. Divalent cations such as calcium and magnesium, which are often present in the cell culture environment, inhibit this action. EDTA sequesters these ions and thus enhances the efficacy of…
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Dulbecco's Modified Eagle's Medium (DMEM), High Glucose, with L-Glutamine
Quality BiologicalDMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells. We offer a variety of DMEM modifications…
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Minimum Essential Medium (MEM) is a common cell culture medium developed from early work using Basal Medium Eagle (BME) with normal mammalian fibroblasts and certain subtypes of HeLa cells. This research indicated that additions made to BME aided cell propagation. MEM is modified with…
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All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt…
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Catalase Reagent Droppers are used in a qualitative procedure for determining catalase activity by bacteria. The enzyme catalase is preset in most aerobic and facultatively anaerobic bacteria, with the major exception of streptococci and enterococci. The enzyme catalase decomposes hydrogen…
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This Tryptic Soy Broth (TSB) product is offered for use in sterility testing and validation in the manufacture of a liquid product. The use of this medium in place of the product intended for production allows an accurate assessment of sterility throughout the manufacture process. The product is a…
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Poly-D-Lysine (PDL) and Poly-L-Lysine (PLL) are synthetic compounds that enhance cell adhesion and protein absorption by altering surface charges on the culture substrate. In addition to promoting cell adhesion, poly-lysine surface treatments support neurite outgrowth and improve the survival of…
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Water, Sterile, Tissue Culture Grade. Used for reconstitution of powdered media and other cell culture reagents.
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20X SSPE
Corning20X Saline-Sodium-Phosphate-EDTA (SSPE) buffer has applications in nucleic acid hybridization and other molecular biology work.
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Collagen I, found in most tissues and organs, is most plentiful in dermis, tendon, and bone. It is an integral part of the framework that holds cells and tissues together and has been recognized as a useful matrix for improving cell culture. in vitro use of collagen can exert effects on the…
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RPMI-1640 with L-Glutamine
Quality BiologicalRPMI was developed to support lymphoblastoid cells in culture, RPMI-1640 has now been successfully used for the culture of a wide range of anchorage independent cells. Although intended for use with serum supplementation, RPMI-1640 has been shown to support the serum-free growth of a number of…
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Thomas Nos. B003K38 and B003K39 Iscove's Modification of DMEM (IMDM) is highly enriched synthetic medium optimized for rapidly proliferating cultures of high cell density. A modified form of Dulbecco's Modified Eagle Medium (DMEM), Iscove's contains additional amino acids,…
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Poly-D-Lysine (PDL) and Poly-L-Lysine (PLL) are synthetic compounds that enhance cell adhesion and protein absorption by altering surface charges on the culture substrate. In addition to promoting cell adhesion, poly-lysine surface treatments support neurite outgrowth and improve the survival of…
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PBS (Phosphate Buffered Saline) Tablets
Bio Basic Inc.PBS (Phosphate buffered saline), 100 ml tablets: PBS (Phosphate buffered saline), 100 ml tablets Phosphate Buffered Saline tablets have been specifically developed for use in immunoassay procedures. Reconstitution: One tablet dissolved in 100 ml of deionized water yields 1 X PBS…
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Standard Aerobic Culture Medium in Plastic Culture Vials
BECTON DICKINSON AND COMPANY(Continental US Only) (Must be kept Refrigerated; Ships Overnight and on Ice) (Drop Ship Requires Pre-Approval)
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Bio-Rich 1 (DMEM/F12 Enriched) with L-Glutamine
MP BiomedicalsBioRich 1 with 10 common anchorage dependent cell lines commonly show increased yields from 8% to 114% when compared to standard media in normal serum-containing systems. BioRich 1 also shows elevated growth of hybridoma lines at reduced serum concentrations when compared to normal base media.…
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In many cell culture applications using defined media for the in vitro cultivation of cells, the addition of supplemental nutrients and reagents is required. Additives such as antibiotics, buffers, and stains are frequently used to prevent bacterial contamination, control pH, and visibly monitor…
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CMRL media are based on the composition of Medium 199. Nucleic acid derivatives, coenzymes omitted in H597 modification were retained in CMRL 1066. CMRL 1066 is a chemically defined medium formulated for long term cultivation of mouse L cells in the absence of protein supplement. Although…
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Technical Advantage: Product manufactured under industry-leading standards for low endotoxin content and superior results Minimum Essential Medium (MEM) is a common cell culture medium developed from early work using Basal Medium Eagle (BME) with normal mammalian fibroblasts and certain…
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Leibovitz' L-15 Medium without L-Glutamine
Quality BiologicalDesigned for cell culture without CO2. Without Sodium Bicarbonate With Phenol Red Without L-glutamine Applications L-15 has been used for the culture of HEp-2 monkey kidney and primary explants of embryonic and adult human tissue, and a variety of other cells. Many viruses have also been…
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Minimum Essential Medium Eagle
MP BiomedicalsPreparation Method: Add 5% less distilled water than the desired total volume to a mixing container just large enough to hold the final media volume. Add powdered medium to room temperature (20 -30°C) water with gentle stirring. Do not heat water. Rinse inside of pouch to remove all traces of…
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Alpha MEM with L-Glutamine
Quality BiologicalAlpha MEM is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. Recommendation This product is made with Earle’s salts. MEM a contains no proteins, lipids, or…
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Poly-D-Lysine, 20 mg
CorningPoly-D-Lysine, used to culture a wide variety of cell types, particularly neurons, glial cells, and transfected cells. (20 mg) Source: Synthetic Quality: Shown to promote cell attachment and spreading of rat cerebellar granule cells Filtered (0.2 µm membrane) and tested and found negative…
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The Corning portfolio of cytokines, growth factors, and media supplementation offers a wide array of proteins that result in proliferation or differentiation of the affected cells. Vialed, highly purified growth factors with high biological activity Broad range of highly purified natural and…
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Nutrient Mixture F-12 Ham, Coon’s Modification w/Sodium bicarbonate w/o L-Glutamine.
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Prepared Culture Media (GMBCP)
CrosstexCrosstex Prepared Culture Media consists of an exclusively formulated Trypticase® Soy Broth (TSB) modified with pH indicator. A reduced incubation time of 24 hours for steam and hydrogen peroxide (VH2O2) sterilization has been validated when Crosstex Biological Indicator Spore Strips and Discs…
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HiGlutaXL™ Dulbecco’s Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM / F12, 1:1 Mixture) w/ L-Alanyl-L-Glutamine, HEPES buffer, Sodium bicarbonate and Trace elements. Contains stabililzed dipeptide of L-Glutamine.
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Hanks' Balanced Salt Solution 1X w/ Phenol red and Sodium bicarbonate w/o Calcium. Used with cells maintained in less CO2 environment or CO2 free environment.
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Dulbecco's Phosphate Buffered Saline 1X w/ Calcium and Magnesium w/o Phenol red. Commonly used for tissue disaggregation and monolayer dispersal.
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Water, Sterile, Molecular Biology Grade Endotoxin tested, DEPC treated, Nuclease and Protease free. Used for molecular biology applications like DNA/RNA purification, protein applications and experiments involving RNA.