Tissue Culture Media
The primary purpose of tissue culture media is to provide a synthetic environment within the lab. This synthetic environment then allows cells to thrive and carry out normal functions.
Ideal for primary cell isolation and cell line maintenance, culture media is essential for the isolation and propagation of primary cells obtained from tissues.
PBS cell culture media is recommended for use when investigating the localization and distribution of target proteins in different parts of biological tissue.
Tissue culture media accessories include reagent-grade water, antibiotics, and Corning BioCoat cell culture inserts to assist you with achieving clear and accurate results.
-
CDC Anaerobe 5% Sheep Blood Agar is used for the isolation and cultivation of fastidious and slow growing, obligately anaerobic bacteria from a variety of clinical and nonclinical materials. It also supports good growth of most aerobic, facultatively anaerobic and microaerophilic bacteria if…
-
HEPES is a zwitterionic organic buffering agent. It is stable, water soluble and does not react with divalent cations. It has a pKa of 7.5 at 37deg C and offers an optimal buffering range of pH 6.8 - 8.2. It maintains the physiological pH despite changes in the carbon dioxide concentrations which…
-
2X EMEM without Phenol Red
Quality BiologicalEagle’s minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Basal medium with different variations Without Phenol Red With Glucose and L-glutamine Applications EMEM (Eagle’s Minimum Essential Medium) has…
-
RPMI 1640
CorningNamed for the site at which it was developed, the Roswell Park Memorial Institute, RPMI 1640 is a modification of McCoy's 5A containing an increased level of inositol. Examples of cell types for which this medium is appropriate include mammalian myeloma and hybridoma cells such as mouse…
-
Corning® BioCoat™ Gelatin Plates
CorningCorning BioCoat Gelatin cultureware provides an attachment and growth promoting substrate for the culture of a variety of cell types. Gelatin is commonly used in the culture of vascular endothelial cells, muscle, embryonic stem (ES) cells, and F9 teratocarcinoma cells. It is also suitable for…
-
Para-Marker
Electron Microscopy SciencesA wide tipped pen, designed for use with paraffin sections. The pen provides a sticky membrane to the glass slide which not only prevents paraffin sections from falling off but also keeps the sections flat on the slide.
-
Corning® Medium 199 (Mod.)
CorningMedium 199 is often used in vaccine production, virology, and for the culture of various non-transformed cells. It was originally developed as the result of efforts to create a fully defined, animal origin-free medium. Long-term use of this medium requires serum supplementation. This formulation…
-
Tryptose Phosphate Broth 50X
HiMediaTryptose Phosphate Broth 50X. Used to enhance the growth supporting property of GMEM for BHK-21 cells. It is also used in insect cell culture media to support the growth of SF-21 cells.
-
-
Cellvation Cryopreservation Media
MP BiomedicalsCellvation Cryopreservation Media is animal component free and has been shown to preserve the potential of stem cell for differentiation. This medium is recommended for the preservation of stem cells, hepatocytes, tissue samples, and a wide variety of cell types and established cell lines. …
-
The Corning® Mediatech™ cell culture media is formulated with high industrial standards for excellent results. It maintains cell tonicity and viability. Application: For Research Use Only
-
This Tryptic Soy Broth (TSB) product is offered for use in sterility testing and validation in the manufacture of a liquid product. The use of this medium in place of the product intended for production allows an accurate assessment of sterility throughout the manufacture process. It is a sterile,…
-
Corning insectagro Sf9 is formulated to support the propagation of Sf9 insect cells in culture, and can also be used for SF21 cells. The Sf9 cell line originated at the USDA Insect Pathology Laboratory from IPLBSF-21 cell line, derived from the pupal ovarian tissue of the fall army worm. Sf9 cells…
-
Corning BioCoat cell culture inserts are pre-coated with extracellular matrix proteins for applications requiring a protein-coated cell surface, such as cell adhesion, growth, invasion, migration and/or differentiation. Coatings include Corning Matrigel® matrix, Fibronectin, Collagen, and Laminin.
-
Fro-Marker
Electron Microscopy SciencesA wide tipped pen designed for use with frozen sections. The pen provides a sticky membrane which when applied to the slide and onto which the section is placed it eliminates the section from falling off, moving, or wrinkling on the slide during immunostaining procedures. The membrane is stable up…
-
Prepared plated media 100 x 10 mm and 100 x 15 mm plates
-
Buffers
CorningSodium bicarbonate Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7.2 - 7.4 in a culture environment. Bicarbonate is a weak buffer with that makes maintaining a pH range of 7.2 - 7.6 somewhat difficult, but it is non-toxic, and is a…
-
PBS (Phosphate Buffered Saline) Tablets
Bio Basic Inc.PBS (Phosphate buffered saline), 100 ml tablets: PBS (Phosphate buffered saline), 100 ml tablets Phosphate Buffered Saline tablets have been specifically developed for use in immunoassay procedures. Reconstitution: One tablet dissolved in 100 ml of deionized water yields 1 X PBS…
-
HTS insert plates are arrays of individual cell culture inserts connected by a rigid, robotics-friendly holder. This single-unit design makes insert plates ideal for running automated, high throughput drug transport (Caco-2 cells) cell toxicity studies or cell migration and invasion studies. …
-
Liquid Blocker–Super PAP Pen
Electron Microscopy SciencesThe Newest Staining Guard Pen New and improved. The Super PAP Pen outperforms the original PAP Pen. The Super PAP Pen may be used at temperatures as high as 120°F. (The old style PAP Pen worked only up to 90°F). With this high temperature feature, one can now use the Super PAP Pen to…
-
Corning Matrigel basement membrane matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in ECM proteins. Its major component is laminin, followed by collagen IV, heparan sulfate proteoglycans, entactin, and nidogen. Corning…
-
Human Fibronectin (HFN) is a widely distributed glycoprotein that is used as a substrate to promote attachment of cells through its central-binding domain RGD sequence. HFN is a product of most mesenchymal and epithelial cells and is present in both the ECM and plasma. The principal function of HFN…
-
Thomas Nos. B003K38 and B003K39 Iscove's Modification of DMEM (IMDM) is highly enriched synthetic medium optimized for rapidly proliferating cultures of high cell density. A modified form of Dulbecco's Modified Eagle Medium (DMEM), Iscove's contains additional amino acids,…
-
Poly-D-Lysine (PDL) and Poly-L-Lysine (PLL) are synthetic compounds that enhance cell adhesion and protein absorption by altering surface charges on the culture substrate. In addition to promoting cell adhesion, poly-lysine surface treatments support neurite outgrowth and improve the survival of…
-
Minimum Essential Medium (MEM) is a common cell culture medium developed from early work using Basal Medium Eagle (BME) with normal mammalian fibroblasts and certain subtypes of HeLa cells. This research indicated that additions made to BME aided cell propagation. MEM is modified with…
-
For some applications, the use of a combination of ECM proteins, such as Laminin (LM) and Fibronectin (HFN) or LM and attachment factors such as Poly-D-Lysine (PDL) or Poly-L-Orinthine (PLO) has been shown superior to the use of either alone. Corning BioCoat PDL/LM and PLO/LM Cultureware is…
-
Corning® Ham's F-12 Medium
CorningHam's F-12 media was originally formulated for the serum-free culture of CHO cells, and is based on Ham's F-10 medium with increased concentrations of choline, inositol, putrescine, and several amino acids. This medium is also suitable for carcinoma cells, rat skeletal myoblasts,…
-
Yeast Extract
HiMediaYeast Extract is the water-soluble portion of autolyzed yeast. It provides naturally occurring B-complex vitamins, nitrogen, amino acids and carbon in cell culture media. TCL041 is a sterile filtered solution of Yeast Extract. It is most commonly used as an enrichment supplement to grow Mycoplasma.
-
FlexiPump® Pro Complete Dispensing Assembly
InterscienceThe FlexiPump Pro complete dispensing assembly with a Y connector makes it easy to gain access to various liquid samples without the risk of any harmful consequences.
-
Medium 199, originally described by Morgan and his colleagues (1950), is a completely defined nutritional source for cell culture. Their investigations demonstrated that cell growth could be measured in this medium. It has broad species applicability including the culturing of non-transformed cell…
-
Interleukin-2 (IL-2), mouse recombinant, plays a central role in cell-mediated immune response. Was first identified by its ability to promote the long-term proliferation of activated T-cells. (25,000 BRMP units) References: 1. Hatakeyama, M. and Taniguchi, T., "Interleukin-2" in Peptide Growth…