In many cell culture applications using defined media for the in vitro cultivation of cells, the addition of supplemental nutrients and reagents is required. Additives such as antibiotics, buffers, and stains are frequently used to prevent bacterial contamination, control pH, and visibly monitor media conditions. Other supplements such as amino acids and vitamins are useful for enriched media beyond their normal concentrations. Attachment factors and transport factors are added to growth media to facilitate rapid cell propagation and enhance proper cell metabolism. Still other media supplements may be added to create conditions which will elicit specific cellular responses and effects, such as altered protein biosynthesis, accelerated antibody production and secretion, toxic response mechanisms, etc.
May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.
100X Non-essential Amino Acids for Minimum Essential Medium Eagle Contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser.
Suitability: Suitable for cell culture
Key Applications: Growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium
Application Areas: Mammalian Cell Culture
Product Type: Culture Media
Culture Media Type: Mammalian Culture Media
Presentation: Clear, Colorless Solution
Format: Solution
Formulation: L-Alanine: 890 mg/L, L-Asparagine H2O: 1500 mg/L, L-Aspartic Acid: 1330 mg/L, L-Glutamic Acid: 1470 mg/L, Glycine: 750 mg/L, L-Proline: 1150, L-Serine: 1050 mg/L
pH: 2.8 - 3.8
Foreign activity: Mycoplasma: Negative
NOTES: Osmolality: 59 - 89 mM/kg; Without L-glutamine
Storage & Handling: Store at +4°C