- Promotes cell attachment, spreading, proliferation, and differentiation of many cell types, particularly fibroblasts and other mesenchymally-derived cells
Fibronectin exists as a dimer in plasma and in multimeric form in the extracellular matrix and on cell surfaces. The primary function of fibronectin is cell adhesion to the extracellular matrix that occurs through an interaction of the Arg-Gly-Asp (RGD) sequence in its cell-binding domain with fibronectin-specific cell surface receptors. The conformation and orientation of adsorbed fibronectin has been shown to affect cell spreading and strength of adhesion of endothelial cells.
Corning® BioCoat™ Fibronectin Cellware is a line of tissue culture vessels with a uniform application of human fibronectin. This cellware is manufactured in a highly controlled environment and rigorously tested to assure product consistency and performance.
Applications include:
- Promotion of cell attachment and spreading
- Rapid expansion of cell populations
- Serum-free or reduced-serum culture
- Cell adhesion assays
- Studies of effects of fibronectin on cell behavior
- Improving survival of primary cells in culture
Quality Control:
Tested for ability to promote attachment and spreading of BHK-21 hamster kidney cells. Tested and found negative for bacteria and fungi. Fibronectin purity > 90% by SDS-PAG.
Storage and Stability:
Cellware is stable for at least three months at 2-8°C. Do not freeze.