The FastDNA SPIN Kit for Soil is designed to isolate bacterial, fungal, plant, and animal genomic DNA from soil and other environmental samples. Soil is processed by a FastPrep instrument and Lysing Matrix E tubes designed to efficiently lyse all microorganisms. The released DNA is purified by a silica-based spin filter method and is suitable for PCR analysis and other downstream applications. Complete lysis of all organisms, including historically difficult sources such as eubacterial spores and endospores, gram positive bacteria, yeast, algae, nematodes and fungi is achieved within seconds using a FastPrep instrument and Lysing Matrix E tubes.
Key Applications: Used to isolate bacterial, fungal, plant and animal genomic DNA from soil and other environmental samples.
Application Notes: Cited in over 6,000 scientific publications, the FastDNA SPIN Kit for Soil delivers the highest DNA yield from any environmental sample including soil, sediments, sludge, compost, manure, rhizosphere or waste water. The kit contains Lysing Matrix tubes and all the reagents needed to extract DNA in a process that efficiently removes humic acids and other PCR inhibitors.
Components: 50 x 2.0 mL tubes of Lysing Matrix E, 60 mL Sodium Phosphate Buffer, 8 mL MT Buffer, 25 mL of PPS Solution, 66 mL Binding Matrix, 50 Spin Modules, 50 Catch Tubes, 12 mL Concentrated SEWS-M, 20 mL DES, 200 µl BBS Gel Loading Dye
Format: Kit
Hardness: Ceramic Spheres, Vickers 800; Silica Spheres, Knoop 515; Glass Bead, Knoop 515
Sample Type: Seeds, Spores, Bacteria (Gram +/-), Animal Tissues, Feces, Food, Fungi, Plants, Roots, Soil, Wastewater, Yeast
Sample Volume: Up to 500 mg per prep
Specific Gravity: Ceramic Spheres, 4; Silica Spheres, 2.5; Glass Bead, 2.5