BM-2 Terrestrial Orchid Medium has been specially formulated for the in vitro culture of the terrestrial orchids. It is a nutrient blend of inorganic salts, vitamins, amino acids, carbohydrate and gelling agent. In addition, it is supplemented with casein hydrolysate which is ideal for improved germination, early protocorm formation and seedling development. L-glutamine and glycine serve as sources of organic nitrogen. Microelements like Manganese, Molybdenum, Copper, Iron and Zinc enhances the catabolism in plants. Boron plays a key role in carbohydrate metabolism. Thiamine, pyridoxine, nicotinic acid act as enzymatic cofactors in universal pathways including glycolysis and TCA cycle along with primary and secondary metabolism in the plants. 6-BAP helps in the cell division and differentiation of the plant tissue. CleriGel™, a gellan gum is used as an alternative to agar. It offers several advantages over conventional agar as it sets a clear gel which assists easy observation of cultures and their possible contamination. Unlike agar, gel strength of CleriGel™ is unaffected over a wide range of pH and contains no contaminants like phenolic compounds that can be toxic to plant tissues. It solidifies uniformly and rapidly. The product is plant tissue culture tested but it is the sole responsibility of the user to ensure the suitability of the medium for individual species.
Application: For the in Vitro Culture of the Terrestrial Orchid
- Appearance: White to off-white, homogenous, free flowing powder
- Solubility: 24.2 gms/litre soluble after boiling in distilled water
- Color and Clarity: Colorless to light yellow solution, clear gel is formed on cooling
- Gelling: Firm gel formed at pH : 5.75 ± 0.5