Plate Count HiVeg™ Agar with Tween 80 and Lecithin is used for sanitary examination of surfaces, that is, for counts before and after application of disinfectants. Addition of Polysorbate 80 and lecithin act as neutralizers to inactivate the residual disinfectants where the samples are collected. Lecithin inactivates quaternary ammonium compounds whereas polysorbate 80 neutralizes formalin, phenolic disinfectants, hexachlorophene etc. Standard Methods HiVeg™ Agar w/ Tween 80 and Lecithin is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/ TSE risks.
Application: For Examination of Sanitary Quality of Surfaces
- Appearance of powder: Light yellow colored, may have slightly greenish tinge, homogeneous, free flowing powder
- Gelling: Firm, comparable with 1.5% Agar gel
- Color and Clarity: Light yellow colored, slightly opalescent gel forms in petri plates
- Reaction: Reaction of 2.92% w/v aqueous solution is pH 7.0 ± 0.2 at 25°C
- Cultural Response: Cultural characteristics observed after an incubation at 35-37°C for 24 - 48 hours