Nutrient Broth Medium is a general purpose medium used for the examination of water and dairy products according to Standard Methods for the Examination of Water and Wastewater (1) and Dairy Products (2) in accordance with IP (4). It can also be used for cultivating several less fastidious microorganisms.
Beef extract and peptone provide the necessary nitrogen compounds, carbon, vitamins and also some trace ingredients to the non-fastidious organisms. Sodium chloride maintains osmotic equilibrium of the medium.
Directions:Suspend 25 grams in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Sterilise by autoclaving at 10 lbs pressure (115°C) for 30 minutes.