Lactose Broth is recommended by Indian pharmacopoeia for selective pre-enrichment of Enterobacteriaceae as well as for Escherichia coli and Salmonella in water, food and pharmaceutical products. The medium is used for detection of specified microorganisms of non-sterile products according to European Pharmacopoeia (EP). This medium is also recommended by various other pharmacopoeia. Lactose Broth is also recommended by APHA in the performance and confirmation of the presumptive test for coliform bacteria in water, food, milk. This medium can be used as an alternate to Lauryl Sulphate Broth in the presumptive test of the MPN of standard coliforms.