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Hamilton

PRP-X400 Cation Exchange HPLC Columns

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PRP-X400 Cation Exchange HPLC Columns

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Polymeric cation exchange packing for separation of glyphosate and its metabolite in drinking water.

  • Faster analysis when compared to competitor’s columns
  • Detection levels of less than 10 ppb when using the post column, OPA derivatization technique
  • Applicable to other separations, such as inositol and sugar alcohols
  • pH stable from 1 to 13
     
  • Two particle sizes: 7 and 12-20 μm
  • Five column internal diameters: 2.1 to 21.5 mm
  • Two column materials: 316 stainless steel and PEEK
  • Analytical and semiprep/preparative guard columns

The PRP-X400 column provides a fast separation for glyphosate and its metabolites. The exchange capacity of the PRP-X400 is greater than that of the PRP-X200, leading to characteristics better suited for the separation of glyphosate. It also performs well in other separations, such as inositol and sugar alcohols. The column does not have to be heated to 65°C and operates well at room temperature, so a column heater is not necessary for this method. PRP-X400 columns do not require the use of methanol in the mobile phase, and they cost much less than other glyphosate columns.

Pack Your Own Columns - Hamilton offers a range of bulk polymer resins for customers who prefer to pack their own column hardware. Chromatographic capacity and efficiency tests are conducted on both bulk and packed column resins to ensure product integrity. Samples from different lots can be purchased for method and process validation.

Protect Your Investment - Hamilton guard columns protect analytical, semi-prep and preparative HPLC columns. They also remove particulate contaminants and highly adsorptive compounds from samples, prolonging column life. The designs are modular and cartridge replacement is easy and tool-free.  Cartridge holders for analytical columns are available in stainless steel and PEEK materials. Semi-prep/preparative holders are available only in stainless steel. The design of the cartridge holders is optimized to reduce dead volume and prevent extra column band broadening. Cartridge holders are reusable indefinitely.

PRP-X400 Cation Exchange
HPLC Columns, PRP-X400
Thomas No. 1216M16
Column, 7µm 250 x 4.1mm
Stationary PhasePRP-X400
ModeCation Exchange
Packing Material TypePSDVB/Sulfonic Acid
Particle Size7 µm
Pore SizeN/A
FormatColumn
Hardware Inner Diameter4.1 mm
Hardware Length250 mm
Hardware MaterialStainless Steel
Max. Pressure5000
Exchange Capacity2.5 meq/gm
Mobile Phase LimitspH 1-13. 0-100% aqueous, organic modifier
Buffer Strength0.0-0.5N
Temp Limits5-60°C
RestorationInject several times with 100 µL of 0.1 M potassium EDTA
Compound ClassificationGlyphosate
UOM1 Each
Exchange Capacity/Column4.0 meq/column
Analytical Guard Column
Starter Kits and Cartridge
Replacements, PRP-X400
Thomas No. 0470U55
Column Starter Kit C-18
Thomas No. 0470U59
Cartridge Replacement C-18 Pk5
Stationary PhaseHxSil C18HxSil C18
ModeReversed-PhaseReversed-Phase
Packing Material TypeSilicaSilica
Particle Size10 µm10 µm
FormatGuard Cartridge KitReplacement Guard Cartridge
Hardware Inner Diameter2.1 mm2.1 mm
Hardware Length20 mm20 mm
Guard Column SizeAnalytical
Hardware MaterialStainless SteelStainless Steel
Max. Pressure5,000 psi5,000 psi
Mobile Phase LimitspH 2.0 - 7.5pH 2.0 - 7.5
Compound ClassificationGeneral PurposeGeneral Purpose
UOM1 holder, 2 cartridges5/p
Analytical Guard Column
Starter Kits and Cartridge
Replacements, PRP-X400
Thomas No. 1177F72
Analytical Guard Column Starter Kit
Thomas No. 1177FF83
Analytical Guard Column
Stationary PhasePRP-X400PRP-X400
ModeCation ExchangeCation Exchange
Packing Material TypePSDVB/Sulfonic AcidPSDVB/Sulfonic Acid
Particle Size7 µm7 µm
FormatGuard Cartridge KitGuard Column
Hardware Inner Diameter2.1 mm2.1 mm
Hardware Length20 mm33 mm
Guard Column SizeSemiprep/PreparativeAnalytical
Hardware MaterialStainless SteelStainless Steel
Max. Pressure5,000 psi5,000 psi
Mobile Phase LimitspH 1-13. 0-100% aqueous, organic modifier_
Compound ClassificationOrganic/Inorganic CationsGlyphosate
UOM1 holder, 2 cartridges1 Each
Bulk Resin, PRP-X400Thomas No. 1177F84
7 µm Bulk Resin
Thomas No. 1177F845
12-20 µm Bulk Resin
Thomas No. 1177F86
30-50 µm Bulk Resin
Stationary PhasePRP-X400PRP-X400PRP-X400
ModeCation ExchangeCation ExchangeCation Exchange
Particle Size7 µm12-20 µm12-20 µm
Pore SizeN/AN/AN/A
FormatBulk ResinBulk ResinBulk Resin
Max. Pressure5,000 psi5,000 psi5,000 psi
Exchange Capacity2.5 meq/gm2.5 meq/gm2.5 meq/gm
RestorationInject several times with 100 µL of 0.1 M potassium EDTAInject several times with 100 µL of 0.1 M potassium EDTAInject several times with 100 µL of 0.1 M potassium EDTA
UOMper gramper gramper gram

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Product Detail
Thomas No.
0470U55
Mfr. No.
79459
Description
Column Starter Kit C-18
list price/quantitytotal
$0.00
Thomas No.
0470U59
Mfr. No.
79452
Description
Cartridge Replacement C-18 Pk5
list price/quantitytotal
$0.00
Thomas No.
1216M16
Mfr. No.
79473
Description
Column, PRP-X400 7µm 250 x 4.1mm
list price/quantitytotal
$0.00
Thomas No.
1177F84
Mfr. No.
79591
Description
PRP-X400, 7 µm Bulk Resin
list price/quantitytotal
$0.00
Thomas No.
1177F85
Mfr. No.
79592
Description
PRP-X400, 12-20 µm Bulk Resin
list price/quantitytotal
$0.00
Thomas No.
1177F86
Mfr. No.
79593
Description
PRP-X400, 30-50 µm Bulk Resin
list price/quantitytotal
$0.00
Thomas No.
1177F72
Mfr. No.
79224
Description
PRP-X400 Analytical Guard Column Starter Kit (1 holder, 2 cartridges), Stainless Steel
list price/quantitytotal
$0.00
Thomas No.
1177F83
Mfr. No.
79290
Description
Analytical Guard Column, PRP-X400
list price/quantitytotal
$0.00
$0.00 (0 Items)

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