SMTgel (silica-based) columns are specialty SMT columns designed for specific applications including gel permeation (GPC), ion exchange, reversed phase, hydrophobic interaction, hydrophilic interaction, and affinity chromatography.
The packing materials feature modified surface chemistry designed to minimize sample adsorption on the silica substrate. Because SMTgel columns are silica-based, they must be operated within the recommended pH range of 2.0 - 9.0. Detailed operating conditions are described in the information accompanying the columns.
SMTgel RP are available in wide selection of pore sizes, from 300A to 2000A. For example, SMTgel 300RP, 1000RP, and 2000RP are packed with rigid, porous, 300A, 1,000A, and 2,000 A, of chemically modified silica gel with nonpolar ligands, respectively. They can be used for the both normal and reversed phase separation of oligomers and polymers up to 100,000 Da Molecular Weight. SMTgel RP columns are shipped in Acetonitrile/water or methanol/water, which can be exchanged for a wide variety of other organic/water solvents. SMTgel RP columns are ideal for gel permeation chromatography and are stable in solvents having a wide range of polarities. The particles DO NOT swell or shrink as the solvent is changed from common organic solvents like toluene through methanol or other solvents. Furthermore, these columns can be used with polar solvents, such as water or water/methanol mixtures. These columns are available in spherical 5 and 10 um particle sizes. Spherical 5 Åm silica particles provide a minimum of 20,000 plates per 30 cm x 7.8 mm I.D. column. Four pore sizes are available, ranging from an exclusion limit of about 2,000 Daltons for 300RP columns to more than 500,000 Daltons for 2000RP columns.
Four different modifications: