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Hamilton

PRP-X500 Anion Exchange Columns

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PRP-X500 Anion Exchange Columns

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Fast separations and good sample recovery

PRP-X500 is a superficially porous polymeric anion exchange column designed for the separation, purification and isolation of proteins, peptides and DNA/RNA. The methacrylate polymeric coating of the PRP-X500 provides a more hydrophilic surface, preventing hydrophobic interaction sample losses typically seen on other commercially available protein HPLC columns.

The non-porous nature of the packing material improves mass-transfer, shortening run times and improving resolution. Both fast separations and good sample capacity are achievable with PRP-X500 columns.

Pack Your Own Columns - Hamilton offers a range of bulk polymer resins for customers who prefer to pack their own column hardware. Chromatographic capacity and efficiency tests are conducted on both bulk and packed column resins to ensure product integrity. Samples from different lots can be purchased for method and process validation.



PXP-500 Columns
Thomas No. 1192F06
PRP-X500 7 µm
4.6 x 50 mm PEEK
Thomas No. 1192D54
PRP-X500 7 µm
4.6 x 150 mm, PEEK
Stationary PhasePRP-X500PRP-X500
ModeAnion ExchangeAnion Exchange
USP ClassificationUSP L23USP L23
Packing Material TypePoly(methacryl amido-propyl trimethyl ammonium chloride)Poly(methacryl amido-propyl trimethyl ammonium chloride)
Particle Size7 µm7 µm
Pore SizeSuperficially PorousSuperficially Porous
FormatColumnColumn
Hardware Inner Diameter4.6 mm4.6 mm
Hardware Length50 mm150 mm
Hardware MaterialPEEKPEEK
Max. Pressure5,000 psi5,000 psi
Exchange Capacity1.6 meq/gm1.6 meq/gm
Mobile Phase LimitspH 1-13. 0-100% aqueous, organic modifierpH 1-13. 0-100% aqueous, organic modifier
Buffer Strength0.0-2.0N0.0-2.0N
Temp LimitspH 1-7.9 (5-60 °C); pH 8-13 (5-30 °C)pH 1-7.9 (5-60°C); pH 8-13 (5-30°C)
Compound ClassificationLarge Proteins/Labeled DNALarge Proteins/Labeled DNA
UOM1 Each1 Each
Exchange Capacity/Column1.92 meq/column
PXP-X500 Analytical
Guard Replacement
Cartridges and
Starter Kits
Thomas No. 1192D54
PRP-X500 Analytical Guard Column
Replacement Cartridges (5/pk), PEEK
Thomas No. 1192D53
PRP-X500 Analytical Guard Column
Starter Kit (1 holder, 2 cartridges), PEEK
Stationary PhasePRP-X500PRP-X500
ModeAnion ExchangeAnion Exchange
Packing Material TypePoly(methacryl amido-propyl trimethyl ammonium chloride)Poly(methacryl amido-propyl trimethyl ammonium chloride)
Particle Size12-20 µm12-20 µm
FormatReplacement Guard CartridgeGuard Cartridge Kit
Hardware Inner Diameter3 mm3 mm
Hardware Length8 mm 8 mm
Guard Column SizeAnalyticalAnalytical
Hardware MaterialPEEKPEEK
Max. Pressure5,000 psi5,000 psi
Mobile Phase LimitspH 1-13. 0-100% aqueous, organic modifierpH 1-13. 0-100% aqueous, organic modifier
Compound ClassificationLarge Proteins/Labeled DNALarge Proteins/Labeled DNA
Frit MaterialTitaniumTitanium
UOM5/pk1 holder, 2 cartridges
Bulk Resins
PRP-X500
Thomas No. 1177G13
PRP-X500, 7 µm Bulk Resin
Thomas No. 1192G14
PRP-X500, 12-20 µm Bulk Resin
Thomas No. 1192G15
PRP-X500, 30-50 µm Bulk Resin
Stationary PhasePRP-X500PRP-X500PRP-X500
ModeAnion ExchangeAnion ExchangeAnion Exchange
USP ClassificationUSP L23USP L23
Packing Material TypePoly(methacryl amido-propyl trimethyl ammonium chloride)Poly(methacryl amido-propyl trimethyl ammonium chloride)Poly(methacryl amido-propyl trimethyl ammonium chloride)
Particle Size7 µm12-20 µm30-50 µm
Pore SizeSuperficially PorousSuperficially PorousSuperficially Porous
FormatBulk ResinBulk ResinBulk Resin
Max. Pressure5,000 psi5,000 psi5,000 psi
Exchange Capacity1.6 meq/gm1.6 meq/gm1.6 meq/gm
UOMper gramper gramper gram

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Product Detail
Thomas No.
1192F06
Mfr. No.
79474
Description
PRP-X500 7 µm 4.6 x 50 mm PEEK
list price/quantitytotal
$0.00
Thomas No.
1192F32
Mfr. No.
79573
Description
PRP-X500 7 µm 4.6 x 150 mm, PEEK
list price/quantitytotal
$0.00
Thomas No.
1192D54
Mfr. No.
79320
Description
PRP-X500 Analytical Guard Column Replacement Cartridges (5/pk), PEEK
list price/quantitytotal
$0.00
Thomas No.
1192D53
Mfr. No.
79319
Description
PRP-X500 Analytical Guard Column Starter Kit (1 holder, 2 cartridges), PEEK
list price/quantitytotal
$0.00
Thomas No.
1177G13
Mfr. No.
79594
Description
PRP-X500, 7 µm Bulk Resin
list price/quantitytotal
$0.00
Thomas No.
1177G14
Mfr. No.
79595
Description
PRP-X500, 12-20 µm Bulk Resin
list price/quantitytotal
$0.00
Thomas No.
1177G15
Mfr. No.
79596
Description
PRP-X500, 30-50 µm Bulk Resin
list price/quantitytotal
$0.00
$0.00 (0 Items)

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