Protect Your Investment - Hamilton guard columns protect analytical, semi-prep and preparative HPLC columns. They also remove particulate contaminants and highly adsorptive compounds from samples, prolonging column life. The designs are modular and cartridge replacement is easy and tool-free. Cartridge holders for analytical columns are available in stainless steel and PEEK materials. Semi-prep/preparative holders are available only in stainless steel. The design of the cartridge holders is optimized to reduce dead volume and prevent extra column band broadening. Cartridge holders are reusable indefinitely.
Ion exclusion for organic acids and alcohols
Hamilton PRP-X300 columns offer an easy, rapid way to separate closely related alcohols and organic acids. The sulfonated poly(styrene-divinylbenzene) support separates samples via a mixed mode mechanism. Separation on the PRP-X300 is accomplished by three modes:
- Hydrogen Bonding —The attraction and retention of sample compounds by the negatively charged sulfonate group.
- Reversed-Phase —The interaction and retention of the sample compounds by the non-polar polymeric support.
- Ion Exclusion —The process in which ionized samples are excluded from the pores of the support and elute first, while the weakly ionized and nonionic compounds elute later.