The Petroff-Hausser Counter is one piece construction ensuring durability and accuracy, featuring an Improved Neubauer ruling on a single plateau. The Petroff-Hausser Counting Chamber is popular for bacteria and sperm counting.
Usage: Sperm Analysis and Counting Bacteria Counting
Cell-Depth: 0.02mm (1/50 mm)
Ruling Pattern: Improved Neubauer, 1/400 square mm
In order to count the number of sperm per milliliter, dilution is not necessary. To immobilize sperm, there are two recommendations:
- (1) Add a drop of Formaldehyde, OR
- (2) Run semen under hot water, which will kill or immobilize.
Rulings cover 9 square millimeters. Boundary lines of the Neubauer ruling are the center lines of the groups of three. The central square millimeter is ruled into 25 groups of 16 small squares, each group separated by triple lines, the middle one of which is the boundary. The ruled surface is 0.02 mm below the cover glass, so that the volume over a square millimeter is 0.02 cubic mm. Count all cells within this center square millimeter (1mm x 1mm area).