Several components are combined in the UVP anesthesia system in order to regulate and administer a combination of oxygen and isoflurane/sevoflurane gas to the animal. The initial anesthesia is performed in the induction chamber. The animal is then moved to the warming plate inside a UVP iBox darkroom and connected to a nose cone. The warming plate generates uniform temperature conditions which maintain a safe body temperature of the animal during the imaging process. A low profile breathing device on the plate connects to patented valves. The system’s non-rebreathing technology prevents backflow of gases into the darkroom and ensures user safety.