The objective of this experiment is to identify the presence of the single nucleotide polymorphism (SNP) in an amplified segment of the PTC gene that links detection of the characteristic taste of PTC paper. This is a set of five modules that starts with (I) extraction of DNA from buccal cells (II) amplifying the segment that contains the polymorphic nucleotide (III) digestion of the amplified fragment with the restriction enzyme that recognizes the SNP (IV) analysis by gel electrophoresis (V) tasting the PTC paper to confirm the results obtained.
Kit Includes: instructions, primer mix, DNA template, PCR EdvoBead™, DNA size ladder, Restriction Enzyme, Restriction Enzyme Reaction and Dilution Buffers, Lysis buffer, agarose, electrophoresis buffer, InstaStain® Ethidium Bromide, PTC paper
All You Need: thermal cycler, electrophoresis apparatus, power supply, automatic micropipet, microwave or hot plate, waterbath, UV transilluminator
Storage: Some Components Require Freezer Storage