For the highly sensitive detection of glycoproteins following gel electrophoresis (Figure 1) or protein transfer to nitrocellulose membranes (Figure 2).
The kit uses an enhanced Periodic Acid-Schiff (PAS) method for detection of glycoprotein sugars. The supplied oxidizing agent first oxidizes the cis-diol sugar groups to aldehydes. The aldehyde groups react with the sensitive Glyco-Stain Solution forming Schiff bonds and producing strong magenta color band s.
In addition to glycoprotein staining, the kit is supplied with RAPIDstain™ , an enhanced Coomassie stain. RAPIDstain™ can be used after glycoprotein staining to detect non-glycosylated proteins and the use of the stain enhances glycoprotein staining.
The GlycoproteinStaining kit is highly convenient as all the key reagents required for staining are supplied and a unique positive & negative control is included. In addition, the kit allows for the detection of glycosylated and non-glycosylated proteins on a single gel or membrane.
The Glycoprotein Staining kit is supplied with an enhance Coomassie stain, RAPIDstain™ , that enhances the glycoprotein staining as well as staining non-glycosylated proteins (Figure 1B).
The kit is sufficient for 10 mini gels (8 x 8cm) or 20 nitrocellulose membrane (8 x 8cm).
Features
- Specific glycosylated protein staining with optional non-glycosylated protein staining
- Two stains in one kit
- Nanogram level sensitivity
- Stable periodate/Schiff staining
Applications
- Improved detection of glycoproteins
- Stain polyacrylamide gels or nitrocellulose membranes