Alcian Blue 8GX is a cationic dye used to quantitatively determine glycosaminoglycans by forming insoluble complexes in solution. Alcian Blue 8GX can be used to determine proteins based on their Resonance Light Scattering by strongly enhancing the signals. It is useful as a bacterial stain, and as a dye for histiocytes and fibroblasts. Alcian Blue 8GX is used as a heteroglycan stain for neutral, sulfated and phosphated mucopolysaccharides and glycosaminoglycans in tissues such as cartilage and extracellular matrices.
Alcian Blue 8X dye shows ring structure with amidinothiomethyl groups.The dye increases in selectivity when increasing amounts of magnesium chloride are added. In contrast, studies show that when electrolyte concentration is very low, Alcian Blue 8GX has trouble binding to carboxylate or phosphate groups.
Key Applications: Bacterial stain
Application Areas: Molecular Biology
Product Type: Biochemicals
Biochemical Category: Labels, Dyes & Stains
Chemical Class: Labels, Dyes & Stains
UV/Visible Absorbance: λmax (water): 615 ± 5 nm
Presentation: Dark Blue Powder
Format: Powder
Solubility: Soluble in absolute ethanol, cellosolve, ethylene glycol, water (8 mg/ml) or ethanol (1 mg/ml); practically insoluble in xylene
Storage & Handling: Store at Room Temperature (15-30°C).