The Viral RNA Extraction Buffer has been optimized to provide rapid, room-temperature lysis of SARS-CoV-2 viral particles in saliva. The buffer comes as a 4x working solution that can be mixed with virus-containing samples in a qRT-PCR plate. Upon 5 minute incubation at room temperature, viral particles are lysed and RNA is stabilized such that samples are ready for direct analysis via RT-qPCR with no additional cleanup step. The Viral RNA Extraction Buffer is compatible with several one-step RT-qPCR kits, including Sigma's Quantitative RT-PCR ReadyMix (QR0200). Removal of precipitates in saliva via centrifugation prior to testing is recommended for optimal sensitivity.
Synonyms: For use with Reverse Transcription-qPCR; Viral Lysis Solution for RT-qPCR; Viral RNA Extraction
Storage: -20C
Application: The Viral RNA Extraction Buffer is broadly compatible with most nucleic acid detection methods and does not reduce signal when used at working concentration in qRT-PCR. qRT-PCR compatibility has been demonstrated in a 4-fluor multiplex setting consisting of Cy5, TxRed, FAM, and HEX.RT-qPCR compatibility has been verified with several one-step RT-qPCR kits:
•Quantitative RT-PCR ReadyMix(TM) (Sigma-Aldrich, catalog QR0200)
•KAPA PROBE FAST One-Step Universal (Sigma-Aldrich, catalog KK4752)
•KiCqStart(R) One-Step Probe RT-qPCR ReadyMix(TM) (Sigma-Aldrich, catalog KCQS07)
•TaqPathTM 1-Step Multiplex Master Mix (Thermo Fisher, catalog A28525)The Viral RNA Extraction Buffer is also compatible with colorimetric LAMP assays.
Legal Information: ReadyMix is a trademark of Sigma-Aldrich Co. LLC