T7 Endonuclease I, a stable homodimer of identical 149 amino acid subunits (1) is the product of a recombinant gene in E. coli . Double-stranded breaks (DSBs) generated by CRISPR/TALEN at desired target sites can be PCR-amplified, and the PCR products can be denatured and re-annealed to form mismatched DNA. If the mismatched DNA length position is more than 1 bp, T7 endonuclease I can recognize and cleave it. It is useful for quantitatively estimating the nuclease-induced mutation frequency of gene edited cells.
Product Includes:
• T7 Endonuclease I
• 10x T7 Endonuclease I reaction buffer
• Extremely high purity
• Useful to resolve four-way junction or branched DNA, Detect/cleave heteroduplex/nicked DNA, and randomly cleave linear DNA for shot-gun cloning
• Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product.