F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc5µ portion of the human IgM heavy chain but not with human IgG, IgA, or the light chains of human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine serum proteins, but it may cross-react with IgM from other species.
Target: Rat
Host: Goat
Antibody Format: Whole IgG
Specificity: IgG, F(ab')2 Fragment Specific
Minimal Cross Reactivity: Human, Bovine, Horse Serum Proteins
Conjugate: PerCP
Clonality: Polyclonal
This product is for IN VITRO RESEARCH USE ONLY. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.