Primary Nav Button

  • PRODUCT AVAILABILITY: Did you know you can view a product's availability right on the product page? Simply enter the quantity you want to purchase and the current availability will appear below the item.

  • Product C840J58 is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

Jackson Immunoresearch

Peroxidase-AffiniPure Goat Anti-Human IgG (H+L)

Not yet rated

Peroxidase-AffiniPure Goat Anti-Human IgG (H+L)

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule horse IgG. It also reacts with the light chains of other horse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

Target: Human

Host: Goat

Antibody Format: Whole IgG

Specificity: IgG (H+L)

Conjugate: Horseradish Peroxidase

Clonality: Polyclonal

This product is for IN VITRO RESEARCH USE ONLY. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

Physical StateFreeze-dried powder
StorageStore freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Alternatively, aliquot and freeze the product at -70°C or below in the absence of glycerol. Avoid repeated freezing and thawing. 
Expiration dateOne year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
PurityThe antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed. 
Buffer0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6  
Stabilizer15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) 
Preservative0.05% Sodium Azide 
Suggested Working Concentration or Dilution Range
ELISA1:5,000-1:100,000
Western Blot1:5,000-1:100,000 (non-ECL) / 1:10,000-1:200,000 (ECL)
Histo-/Cyto-Chemistry1:500-1:5,000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Please Enter Your Order Info

Filter by:

  • Clear Filters
Product Detail
Thomas No.
C840J58
Mfr. No.
109-035-003
Description
Peroxidase-AffiniPure Goat Anti-Human IgG (H+L), 2 mL
list price/quantitytotal
$0.00
$0.00 (0 Items)
Product C840J58 is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

May we suggest

Write A Review





Submit Review
Cancel
Back To Top

About

Resources

Support

Shopping Tools

ISO 9001:2015 Certified Company

©Thomas Scientific 2024 All Rights Reserved. Terms & Conditions