igScript™ Reverse Transcriptase (igScript RT) is a recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase with reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates. igScript RT is an RNA-directed DNA polymerase which lacks 3´ → 5´ exonuclease activity and is capable of producing cDNA from as little as 50 pg of total RNA for real-time RT-PCR analysis and other applications.
Product Includes:
igScript™ Reverse Transcriptase
10x igScript™ RT Reaction Buffer
• Recombinant MMLV reverse transcriptase with greatly reduced RNase H activity.
• Active at temperatures up to 55°C.
• Highly efficient at producing full-length cDNA from as little as 50 pg of total RNA.
• Useful for First strand cDNA synthesis for PCR or RT-PCR and gene expression data validation by using RT-PCR.
• Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product.