HEPES is a widely used buffer in biological studies. In cell culture media, it is employed as a substitute for the bicarbonate buffer at a concentration of 25 mM or as a supplement to the bicarbonate buffer (concentration 10-15mM). The addition of 20mM HEPES to TBE buffer improves the migration behavior of certain samples in the SSCP analysis (single-strand conformation polymorphism), with a higher resolution especially if small differences in the sequences between certain bands are wanted. For research use.
- Molecular Weight: 260.29
- Molecular Formula: C8H17N2NaO4S
- CAS: 75277-39-3
- Purity: >99.0%
- Storage: RT