MDL No.: MFCD03456906
Storage: -70C
UNSPSC Code: 12352204
Application: Furin is capable of cleaving precursors of a wide variety of proteins, including growth factors, serum proteins, including proteases of the blood-clotting and complement systems, matrix metalloproteinases, receptors, viral-envelope glycoproteins, and bacterial exotoxins, typically at sites marked by the consensus sequence Arg-Xaa-(Lys/Arg)-Arg.
Biochem/physiol Actions: Furin is a dibasic endoprotease that is localized in the Golgi apparatus. It has a molecular mass of 52.7 kDa. It is responsible for the proteolytic maturation of many precursor proteins in the secretory and endocytic pathways of mammalian cells. Furin cleaves precursor proteins at their paired basic amino acid processing sites. Some substrates of furin include von Willebrand factor, transforming growth factor beta 1 precursor, pro-beta-secretase and proparathyroid hormone.
Biochem/physiol Actions: Furin is a dibasic endoprotease that is localized in the Golgi apparatus. It is responsible for the proteolytic maturation of many precursor proteins in the secretory and endocytic pathways of mammalian cells.
Physical form: Solution in 10 mM MES, pH 7.0 at 25 C, 1 mM CaCl2, 50% glycerol.
Unit Definition: One unit is defined as the amount of Furin that will release 1 pmole of AMC from the fluorogenic peptide Boc-RVRR-AMC in 1 minute at 30 C.
Preparation Note: Isolated from Spodoptera frugiperda (Sf9) cells infected with recombinant baculovirus carrying truncated human furin
RIDADR: NONH for all modes of transport
WGK Germany: 1