The Oxyrase Enzyme System is obtained from the cytoplasmic membrane of E, coli
Synonyms: EC-Oxyrase®
Storage: -20C; -20C
Application: Various applications of Oxyrase include the preparation of anaerobic media for culture of anaerobic microorganisms, and the protection of oxygen-sensitive materials. In studies of biological processes, Oxyrase can help to enhance the intensity and longevity of activated fluorescent dye which are at risk of oxygen quenching.
Biochem Physiol Actions: EC-Oxyrase requires substrate to be active. Levels at 10 mM to 20 mM are adequate. Substrates for EC Oxyrase include lactic acid, succinic acid, formic acid or their salts, and alpha-glycerol phosphate inaddition to oxygen. EC Oxyrase also contains L-sodium lactate at 100 mM to stabilize the Oxyrase during freeze-thaw cycles. This amount of substrate is diluted to extinction upon use. The exact volume of EC Oxyrase and substrates needed to reduce oxygen in a given system are determined by a number of parameters: including pH, temperature, kinds and amounts of substrates present, surface to depth ratio of the container, and headspace volume. Some experimentation may be necessary; a suggested use level is a 1:100 dilution of the product.
Legal Information: Oxyrase is a registered trademark of Oxyrase, Inc.