Storage: 2-8°C
UNSPSC Code: 12352200
Components: Anti-DNA Antibody (clone MCA-33); Anti-BrdU Peroxidase antibody, Fab Fragments (clone BMG 6H8); Coating Buffer; Washing Buffer; Incubation Buffer; Substrate Solution; BrdU Labeling Reagent; Adhesive Cover Foils
RIDADR: NONH for all modes of transport
Features and Benefits:
• Nonradioactive assay system
• Sensitive (1 x 103 cells/well)
• Low assay background
• No additional dilution steps required
• No washing of the cells required
• Results obtained correlate to those obtained by standard methods
• Denaturation/fixation of DNA in the microplate by microwave irradiation
• Fast (4.5 - 5.5 hours)
Application:
For research use only. Not for use in diagnostic procedures.
The Cellular DNA Fragmentation ELISA is for the quantification of BrdU-labeled DNA fragments either released from cells during necrosis or cell-mediated cytotoxicity, or within the cytoplasm of apoptotic cells.
General description:
The Cellular DNA Fragmentation ELISA measures apoptosis, necrosis, or cell-mediated cytotoxicity by quantifying the fragmentation and/or release of BrdU-labeled DNA. The kit detects DNA fragments: • In the cytoplasm of apoptotic cells, thus providing a nonradioactive alternative to the [3H]-thymidine-based DNA fragmentation assay.
• Released into the culture supernatant during cell-mediated cytotoxicity, thus providing a nonradioactive alternative to the [3H]-thymidine- and [51Cr]-release assays.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Preparation Note:
Working solution: Solubility: soluble in water, aqueous buffers, and dimethylformamide. Can be dissolved in methanol. A clear solution is achieved at 20 mg/ml.
Storage conditions (working solution): Storage of coated plates: 1 week at 2 to 8 °C.
The anti-BrdU-POD stock solution is stable at 2 to 8 °C for several months; for long- term storage it is recommended to store the solution in aliquots at -15 to -25 °C;
The anti-BrdU-working solution should not be stored.
For the anti-DNA-clone MCA-33 we do no have stability data. However it should be no problem to aliquot and store this non-conjugated antibody at -15 to -25 °C for longer storage.