Total RNA Minipreps from tissues, cultured cells and blood samples
The Total RNA MINI and MAXI Kits (Tissue) are specially designed for purification of total RNA from a variety of animal tissues or cells. The provided micropestle can efficiently homogenize tissue samples in the microcentrifuge tube. The method uses a detergent and chaotropic salt to lyse cells and inactivate RNase. The RNA in the chaotropic salt is bound by the glass-fiber matrix of the spin column. Once any contaminates have been removed following the wash step, the purified RNA is eluted by RNase-free water. This protocol does not require phenol extraction or alcohol precipitation. The entire procedure can be completed within 60 minutes, and the purified RNA is ready for RT-PCR, Northern blotting, primer extension, and cDNA library construction.
| Sample Size: | MINI
up to 25mg of tissue / 5x106 of cultured animal cells
MAXI
up to 200mg of animal tissue / 107x108 of cultured animal cells
up to 5ml of blood / 109-1010 of bacteria cells |
| Format: | Spin Column |
| Operation: | Centrifuge / Vacuum Manifold |
| Binding Capacity: | MINI - 60µg / MAXI - 500µg |
| Operation Time: | MINI - 30 minutes /
MAXI - 60 minutes |
| Applications: | RT-PCR, Real-Time RT-PCR, Northern Blotting, Primer Extension, RNase
Protection Assays, mRNA Selection, cDNA Synthesis |
Kit Components
| Component | IB47310 | IB47311 | IB47300 | IB47301 | IB47302 |
| RB Columns | 10 MAXI | 2 MAXI | 4 MINI | 50 MINI | 100 MINI |
| Filter Columns | 10 | 2 | 4 | 50 | 100 |
| 2mL Collection Tubes | - | - | 8 | 100 | 200 |
| Micropestle | - | - | 4 | 50 | 100 |
| RBC Lysis Buffer | 200mL | 35mL | - | - | - |
| RB Buffer | 60mL | 12mL | 2mL | 30mL | 60mL |
| RT Buffer | 30mL | 5mL | - | - | - |
| W1 Buffer | 50mL | 10mL | 2mL | 30mL | 50mL |
| Wash Buffer (add Ethanol) | 25mL (100mL) | 5mL (20mL) | 1mL (4mL) | 12.5mL (50mL) | 25mL (100mL) |
| RNase-Free Water | 6mL | 1mL | 1mL | 6mL | 15mL |