The FastStart High Fidelity PCR System is a blend of FastStart Taq DNA Polymerase and a thermostable proofreading protein, which is also chemically modified. This protein mediates proofreading activity, but carries no polymerase activity. Both proteins are inactive below +75°C and are activated by heating to +95°C for two minutes.
Hot start protocols have improved specificity, sensitivity, and PCR yield. Heat-labile blocking groups on amino acid residues of FastStart Taq DNA Polymerase make the enzyme inactive at room temperature. There is no elongation at low temperatures when primers bind nonspecifically. FastStart Taq DNA Polymerase is activated by removal of blocking groups using a preincubation step at +95°C. The FastStart High Fidelity PCR System is the product of choice for multiplex PCR.
- FastStart High Fidelity Enzyme Blend, (5 U/μl) in storage buffer
- FastStart High Fidelity Reaction Buffer, 10x concentrated, with 18 mM MgCl2
- FastStart High Fidelity Reaction Buffer, 10x concentrated, without MgCl2
- MgCl2 Stock Solution, 25 mM
- DMSO
Features and Benefits
- Achieve excellent multiplex performance
- For fast high quality results, also use Roche′s PCR Optimization Kit
- Increase fidelity
- Achieve fourfold higher fidelity compared to Taq DNA Polymerase and FastStart Taq DNA Polymerase
- Amplify challenging DNA
- DMSO, a PCR additive, for working with difficult templates, is supplied
Packaging
- 1 kit containing 5 components