Circlegrow media is used for growth of E.coli and extends plasmid production past log phase.
Molecular Biology Certified
- Rapid growth of E. coli
- High yields of plasmid DNA
At the end of log phase, cells growing in Circlegrow begin to utilize a proprietary second carbon source that becomes available on a delayed basis. Cell growth and plasmid replication continue long after cells in conventional media have reached stationary phase or expired. Bacteria commonly grow in CIRCLEGROW to a density triple that achieved in other media. The slow post-log phase growth of bacteria in Circlegrow eliminates the need for constant OD monitoring. Circlegrow cultures left overnight or over the weekend will have a much lower percentage of dead cells, and a higher percentage of plasmid DNA, than cultures prepared with any other media. The result is a higher yield of plasmid DNA in less volume with less attention.
Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in Circlegrow formulations. Chosen reagents are combined in the accurate proportions according to the proprietary Circlegrow formula and thoroughly blended for a uniform distribution.