PCRBIO HS VeriFi™ Mix is a versatile and robust proofreading enzyme with AptaLock™ hot start technology for highly precise PCR. Improved DNA binding and increased processivity give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates.
Features of PCRBIO HS VeriFi™ Mix- High temperature cycling – up to 100°C denaturation to better separate GC-rich templates
- Increased PCR success rates with complex genomic templates 17.5kb and over
- 100x higher fidelity than Taq DNA polymerase
- Efficient, specific amplification from challenging templates including GC and AT rich sequences
- High yields under standard and fast PCR conditions (10-30s/kb)
- High temperature cycling for reduced GC bias
- Available as a convenient 2x ready mix
- Generates blunt-end PCR products
Applications- High fidelity PCR
- Long range PCR
- Site-directed mutagenesis
- Cloning
- Sequencing