This special formulation of cloned Pfu DNA polymerase increases PCR product yields up to 19 kb.
Robust performer with very low error rate (six times more accurate than Taq)
Contains ArchaeMaxx factor for high-performance up to 19 kb for gDNA and 15 kb for vector DNA
Available as a HotStart enzyme and in a master mix format
Requires shorter extension times, fewer PCR cycles and less DNA than Pfu DNA polymerase
Blunt ends
PfuTurbo DNA Polymerase is a special formulation of cloned Pfu DNA polymerase and patented ArchaeMaxx Polymerase- Enhancing Factor that has been shown to increase PCR product yields up to 19 kb without affecting replication fidelity.
The ArchaeMaxx factor improves the yield of products by overcoming dUTP poisoning, which is caused by dUTP accumulation during PCR through dCTP deamination. Once incorporated, dU-containing DNA inhibits Pfu and the ArchaeMaxx factor functions as a dUTPase, converting poisonous dUTP to harmless dUMP and inorganic pyrophosphate. The PfuTurbo DNA Polymerase exhibits a lower error rate than other proofreading enzymes and a six-fold lower rate than Taq DNA polymerase.
This product is only available to U.S. Domestic Customers.
For research use only, not to be used in diagnostic procedures.