The EZgene™ Tissue RNA Kit plus combines the reversible binding properties of HiBind RNA technology with a specially designed buffer system which can effectively remove DNA before RNA isolation. Samples are first lysed and homogenized in a specially designed denaturing buffer, which immediately inhibits the activity of RNase and DNase. The lysate is then passed through a EZgene DNA Clearance column which traps the genomic DNA. The flow-through lysate is then applied to RNA column to bind RNA. After two wash steps, purified RNA is eluted with RNase-free water. The whole protocol can be completed in less than 30 minutes. RNA purified using the DNA/RNA method is ready for applications such as RT-PCR, qPCR, differential display, microarrays, etc.