EZgene™ M13 isolation kit allows rapid and reliable isolation of high-quality M13 phage DNA from appropriate host strain. The system combines the reversible binding properties of our matrix with the speed and versatility of spin column technology to yield approximately 3 to 10 μg M13 phage DNA from 1-3 mL of phage supernatant.
In this procedure, the infected bacterial culture is centrifuged and Buffer PC is used to precipitate the phage particle supernatant and then the sample is loaded to the column. The specially designed matrix will allow the phage particles to remain intact on the surface of the matrix. The phage particles are then lysed and bounded to the matrix with the addition of Buffer PB. The pure M13 DNA is eluted with Elution Buffer or ddH2O while unwanted proteins and other impurities are washed away by DNA Wash Buffer.